C－myc和P^（53）卵巢上皮交界性肿瘤中的表达

Expression of c-myc protooncogene and P￣(53) tumor suppressor gene inborderline ovarian epithelial tumors

目的：研究卵巢上皮性交界性肿瘤的发生和发展，以及与卵巢上皮良恶性肿瘤之间的关系。方法：应用地高辛标记ｃ－ｍｙｃ和Ｐ￣（５３）基因探针对２０例良性、２４例交界性、２３例恶性卵巢上皮肿瘤进行原位杂交。结果：ｃ－ｍｙｃ和Ｐ￣（５３）在三种肿瘤组织中表达率分别为１０％，６６．７％，７８．２％和５％，５８．３％，７３．９％，在交界性肿瘤中的表达率介于良恶性肿瘤之间，而且阳性表达率和表达强度与肿瘤组织类型无关，与临床期别和病理分级有关，二者均定位于细胞核内。结论：结果表明卵巢上皮交界性肿瘤的发生及发展可能与ｃ－ｍｙｃ和Ｐ￣（５３）过量表达有关，并可将卵巢上皮交界性肿瘤视为癌前病变。

Objective:To investigate the formation and development of borderline ovarian epithelial tumor atmolecular level and the relationship between the borderline ovarian tumor and benign/malignant ovarian tu-mor.Methods:Digoxigenin- labelled c-myc and p￣(53) gene probes were used in studying the expression of c-mycand p￣(53) genes in the paraffin embedded tissue sections of 24 cases of borderline ovarian epithelial tumors, 20cases of benign ovarian epithelial tumors, 23 cases of invasive ovarian epithelial carcinomas and 12 normal o-varies by in situ hybridization. Results:No expression of the genes appeared in normal ovaries while in thebenign,borderline and invasive epithelial ovarian tumors,the rate of expression of c-myc and p￣(53) mRNA were10%,66.7%,78.2%and 5%,58.3%,73.9%, respectively,the expression of the genes of the borderlinetumor being intermediate between the benign and malignant tumors. The c-myc mRNA and p￣(53) mRNA ex-pressions were both localized in the nuclei with their intensities in close association with the degree of differ-entiation and clinical stage of the tumor, irrespective of their histologic types.Conclusion:The resultsshowed that the origin and development of ovarian epithelial tumors may be associated with over-expressionof c-myc and p￣(53) genes.The borderline ovarian epithelial tumor may be looked upon as cancerous lesion.