γ－氨基丁酸A型受体β_1亚单位基因的分离、克隆及cDNA序列分析

Isolation,cloning and cDNA sequencing of GABA-A receptor β-1 subunit

报道了大鼠大脑γ－氨基丁酸Ａ型受体β－１亚单位基因的分离、克隆，ｃＤＮＡ序列以及相应的氨基酸序列。结果表明在４４９个氨基酸残基长度的成熟多肽链中含有４个跨越细胞膜的疏水性肽段：Ｍ－１，Ｍ－２，Ｍ－３和Ｍ－４；在Ｍ－３与Ｍ－４之间的细胞内肽段，含有ｃＡＭＰ依赖性丝氨酸磷酸化序列：Ａｒｇ－Ａｒｇ－Ａｒｇ－Ａｌａ－Ｓｅｒ；此部位与蛋白激酶Ａ的活性有关，可能与ＧＡＢＡ－Ａ受体被激动后，其信息在细胞内传递的调控有关。

Cloned cDNA encoding the rat β-1 subunit of GABA-A receptor has been isolated.The cDNA sequenceand the deduced amino acid sequence is presented. The predicted 449 amino acid long mature protein contains 4 postulated membrane-spanning hy-drophobic sequences :M-1,M-2,M-3 ,and M-4; between M-3 and M-4 ,there is a cAMP-dependent serine phos-phorylation consensus sequence : Arg-Arg-Arg-Ala-Ser.This site may be related to the activity of proteinkinase A and to the intracellular signal after the activation of the GABA-A receptor.