rhTGF－β_1对人白血病细胞系诱导分化的超微结构研究

Effects of rhTGF-β_1 on ultrastructure of human leukaemia cell lines

以ＨＬ＿（６０）、Ｕ＿（９３７）细胞系为体外模型，应用透射电子显微镜技术，观察了重组人转化生长因子β＿１（ｒｈＴＧＦ－β＿１）对以上两种人白血病细胞系超微结构的影响，结果显示：Ｕ＿（９３７）细胞对ｒｈＴＧＦ－β＿１的反应比ＨＬ＿（６０）细胞敏感，经ｒｈＴＧＦ－β＿１６ｎｇ／ｍｌ作用４天后，Ｕ＿（９３７）细胞之胞核不规则，核仁减少或消失，出现较多的粗面内质网，胞浆中可见吞噬小体，进一步证实ｒｈＴＧＦ－β＿１可诱导Ｕ＿（９３７）细胞向成熟分化，分化途经仍为单核巨噬细胞系。

eukaemia cell line(HL_(60),U_(937))was used as an in vitro model in our study. We investigated the effectsof rhTGF-β_1 on ultrastructure of the two cell lines with transmission electron microscopy. The results showthat the susceptibility of U_(937) to TGF-β_1 was higher than that of the HL_(60) cell.Four days after the inductionof U_(937) cells by TGF-β_1 6ng/ml the size of individual cells was enlarged and the shape of nuclei was irregu-lar.Decrease in number or disappearance of nucleoli was noted. The rough endoplasmic reticula increasedmarkedly in number,There were phagocytic bodies in the cytoplasm. The findings of the present sttidydemonstrate that U_(937) cells could differentiate into mononuclear macrophages following the induction byTGF-β_1.