摘要
将人肝细胞生长因子（ＨＧＦ）ｃＤＮＡ（２．２ｋｂ）经ＤＮＡ连接酶插入到ｐＥＥ１４质粒的多克隆位点上，构建了ｐＥＥ１４／人ＨＧＦ（１１．４５ｋｂ）真核细胞表达质粒。用Ｌｉｐｏｆｅｃｔｉｎ将ｐＥＥ１４／ＨＧＦ质粒转导至ＣＨＯ细胞表达，表达ｐＥＥ１４／人ＨＧＦ的ＣＨＯ细胞的培养上清液能促进原代培养大鼠细胞的ＤＮＡ合成，ＣＨＯ细胞表达和合成的人ＨＧＦ的水平高于２９３和ＮＳＯ细胞。
Institute of Pharrnaceutial Sciences,Guangzhou Institute of Pathology
Case western Reserve University(U.S.A.)The cDNA of human hepatocyte
growth factor(HGF)was inserted into pEE14 plasmid to obtain anew
pEE14 /hHGF plasmid.The pEE14/hHGF plasmid was transfected into CHO
cells by lipofectinmethod.The culture supernatant from the CHO cells
which expressed pEE14/hHGF could stimulate DNAsynthesis in the
primary culture of rat hepatocytes.The CHO cells can express and
secrete higher level ofhHGF than 293 and NSO cells. This is a new
source for preparing hHGF by the expression system of CHOcells.
出处
《第一军医大学学报》
CSCD
1995年第4期321-324,共4页
Journal of First Military Medical University
基金
广东省自然科学基金
关键词
肝细胞
生长因子
质粒
CHO细胞
hepatocyte growth factor
rat
pEE14 plasmid
hepatocyte
CHO
cells
