摘要
为确定纹状体神经细胞膜上存在ATP敏感钾通道,用膜片钳技术在培养新生的大鼠纹状体神经元上记录了单通道电流。在细胞贴附式时,胞外给予NaCN可诱发一种电导为60pS的钾离子单通道,其激活不依赖于膜电位。去极化使通道平均开放时间和开放概率增大。在内面向外式时,ATP或优降糖(glibenclamide)均可抑制通道活动,通道激活不依赖于胞内Ca ̄(2+)。结果表明,大鼠纹状体神经细胞膜上存在一种60pS的ATP敏感钾通道。
n order to
determine whether membranes of striatal neurons contain an
ATP-sensitive K ̄+ Channel,the patch clamp technique was applied to
cultured neurons of rat striatum. In cell-attached
experiments,ex-tracellular application of NaCN elicited a single K ̄+
channel current with a conductance of 60 pS and its ac-tivation was
independent on membrane potential. Depolarization increased the mean
open time and openprobabilty of these channels. In inside-out patch
experiments ,this channel activity was inhibited by an ap-plication
of ATP or glibenclamide to the cytoplasmic surface of the patch
membrane and the channel activa-tion was independent on intracellular
Ca ̄(2+)。These results indicate that the neuronal membranes of rat
stria-tum contain ATP-sensitive K ̄+ channels with a conductance of
60 pS.
出处
《第一军医大学学报》
CSCD
1995年第4期296-299,共4页
Journal of First Military Medical University
关键词
膜片钳
钾通道
纹状体神经元
三磷酸腺苷
Patch clamp
ATP-sensitive K ̄+ channel
striatal
neurons
glibenclamide
rat