大鼠脑损伤早期脑微血管5－HT、DA含量变化及其对外伤性脑水肿的影响

Changes of 5-hydroxytryptamine and dopamine contents in brain microvessels and the influence on cerebral edema at early stage of brain injury

采用高效液相色谱－电化学检测器检测了大鼠脑损伤早期脑微血管中５－ＨＴ、ＤＡ的含量，探讨了二者对外伤性脑水肿的影响。结果显示，脑损伤后０．５ｈ脑微血管中５－ＨＴ、ＤＡ含量以及脑组织水含量均明显增加，至伤后６ｈ仍明显高于对照组（Ｐ＜０．０１）。结果提示，脑微血管中５－ＨＴ、ＤＡ含量明显增高，严重影响脑微血管舒缩功能，损害血脑屏障，可能是外伤性脑水肿发生与发展的重要因素之一。

he contents of 5-hydroxytryptamine( 5-HT)and dopamine(DA)in the brain microvessels(BMVs)at the early stage of rat brain injury were measured by using high performance liquid chromatography withelectrochemical detector(HPLC- ECD),and the influence of the 5-HT and DA level changes on cerebraledema was studied.The results showed that the levels of 5-HT and DA in the BMVs increased apparentlyat 0.5 h ,and were still higher than the control value at 6 h after brain injury(P<0.01).The brain watercontent increased remarkably in the same time,The results indicate that the increment of 5-HT and DA inthe BMVs might be one of the most important factors in the production and development of traumatic brainedema following brain injury.