摘要
本文报道用D-氨基半乳糖所致大鼠暴发性肝衰竭作模型,观察人胎肝细胞对其影响。结果显示,腹腔内注射人胎肝细胞悬液和胞质液均能显著提高肝衰竭大鼠的存活率,两者间比较无显著差别。人胎肝细胞质液可减轻中毒大鼠内毒素血症,降低肝内丙二醛水平,阻止肝坏死,促进肝再生。实验结果提示人胎肝细胞治疗肝衰竭可能主要通过胞质液中的生物活性物质起作用。
The effects of human fetal liver cells on the fulminant hepatic failure induced by D-galactosamiue in rats are observed and the mechanism is investigated.The survival rates of rats injected intraperitoneally with single cell suspension and cytosol are 47.37% and 42.11% and are significantly higher than that of the buffer control(5.26%) (P<0.05),but there is no statistical difference between the two preparations.Administration of cytosol 2 h before intoxication shows that the values of plasma endotoxin and liver malondialdehyde(MDA) in rats are significantly lower(P<0.05,<0.01,or <0.001) and 3H-thymidine incorporation into hepatic DNA(DPM/OD260)higher(P<0.001)than those of the buffer control.The results suggest that some biological active substances in human fetal liver cells may be related to the effects.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1989年第4期266-269,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
肝衰竭
胞质液
人胎肝细胞
liver diseases/CI
galactosamine
cytosol
malondialdehyde
endoloxin
hepatic failure
H-thymidine
