摘要
利用电镜细胞化学方法,定位观察精子发生过程中的Ca ̄(2+)及Ca ̄(2+)-ATPase变化,发现精原细胞的核内外均有弥散性的Ca ̄(2+)分布,精母细胞,圆型精子细胞Ca ̄(2+)主要分布在核膜,线粒体,胞质小囊,内质网,及细胞质膜上;变态期精子Ca ̄(2+)则分布于精子顶体内膜与核膜之间。Ca ̄(2+)-ATPase在精子发生过程中则出现两次,一次是在精母细胞期,另一次是在变态期精子。
By using cytochemistry method to locate Ca ̄(2+) and Ca ̄(2+)-ATPase under electron microscopy during mousespermatogenesis,we found that Ca ̄(2+)irregular distributed in whole of spermatogonium,and appeared on nuclearmembrane,mitochondria,plasma vesicle, endoplasmic reticulum,and plasma membrane;on elongate spermidCa ̄(2+) only located in space between inner acrosomal membrane and nuclear membrane.On the other hand,Ca ̄(2+)-ATPase appeared two times during mouse spermatogenesis,spermatocyte and elongate spermid.
出处
《电子显微学报》
CAS
CSCD
1995年第4期255-260,共6页
Journal of Chinese Electron Microscopy Society
基金
中国科学院"八五"重点资助