HDV阳性乙型病毒性肝炎血清和肝内HBV DNA状态的研究

A Study of the Appearence of HBV DNA in the Serum and Liver of Patients with HDV/HBV Infection

本文报告15例HDV(+)乙型肝炎患者血清和肝内HBV DNA的状态和血清中HBVM的检测结果,并与13例HDV(-)者进行比较。结果发现:血清HBsAg在HDV(+)组有8例滴度≤1:4,其中5例短暂(-),1例住院2天内始终(-),HDV(-)组未见类似现象。HDV(+)和(-)组血清和肝内HBV DNA检出率分别为7/15例(46.7％)与3/12例(25.0％)和7/15(46.7％)与8/13例(61.5％),两者无差异。但HDV(+)组肝内HBV DNA在肝细胞胞浆内分布3例呈稀疏形式,2例着色浅淡,表明HBV复制受限,HDV对HBV可能有抑制现象。本文也具有部份HDV(+)患者肝内HBV DNA复制活跃,3例肝和血清HBV DNA同时阳性,这种结果的不一致可能与HDV感染处于不同阶段有关。

The appearance of HBV DNA in the serum and liver of 15 cases with hepatitis B combined with HDV is described and compared to 13 cases of HDV negative. The results show, titre of HBsAg is less than or equal to 1:4 in 8 cases of HDV positive group, among which 5 temporal negative and 1 negative through 2 d; no similar result in HDV negative group. The detection rates of HBV DNA in HDV positive and negative groups are 46.7%(7/15)and 25%(3/12) in serum and 46.7%(7/15) and 61.5%(8/13) in liver. No statistical difference between them is seen. HBV DNA grains in situ hybridization with biotinylated HBV DNA probe is demonstrated in sparse type of distribution in 3 cases and slightly stained in 2. The HBV DNA replication activity may be supressed and depressed by HDV. Active HBV DNA replication is also present in some HDV positive patients and HBV DNA is positive in liver and serum in 3. It is concluded that different results of HBV DNA detection may be related to HDV infection in different stages.