摘要
α－第二丁基对经基苄醇是正在开发的新型中枢神经系统抑制药物和脑内显影剂。本文建立了该药物的反相高效液相色谱体内分析方法，测定兔血中药物浓度。用无水乙醇沉淀血浆蛋白兼提取药物，浓缩挥干，流动相溶解残渣进样，内标法定量。方法：快速、线性好、选择性强。最小检出量５ｎｇ，回收率９７．６９±４．７９％，日内和日间精密度为２．５３％和３．９３％。血中内源性杂质均不干扰测定。
α-sec-butyl-p-hyedrxybenzyl alcohol is a new chemieal type of sedatives andanticonvulsants ,and a new developing agent in brain. In this poper,RPHPLC assay for determination of itsconcentration in Plasma is established. The method of alcohol precitation plasma egg albumen andsimltaneous extraction drug is simple,rapid and complete without interferences, With vacum- pumping fixedtemporature desieation and condensation,the extractive ratio is up to 97. 69±4.79%,and RSD is 2. 53%within day,3. 93%day to day. The internal standard quantitative method is highly speeific and precise. Theminimum detectable quantity is 5ng.
出处
《电子科技大学学报》
EI
CAS
CSCD
北大核心
1995年第1期102-105,共4页
Journal of University of Electronic Science and Technology of China
关键词
α-第二丁基对羟基苄醇
反相高效液相色谱
药物分析
血药浓度
内标
α-sec-butyl-p-hydroxybenzyl alcohol
reversed-phase HPLC
drugs analysis
drug concentration in plasma
internal standard
