大白鼠黄曲霉毒素慢性中毒的病理组织学研究

PATHOLOGICAL RESEARCH ON AFLATOXICOSIS IN RATS

研究了在慢性中毒期，含黄曲霉毒素玉米（ＡｆＢ、１０００μｇ／ｋｇ）对大白鼠的病理损伤作用．取５２只成年雄性大鼠，分成两个大组，实验（Ｅ）组３５只和对照（Ｃ）组１７只．实验开始后第８周、２０周和４０周分３次扑杀．镜下观察到Ｅ组某些大鼠肾小球系膜基质增厚．系膜细胞增多，用萤光抗体技术证实，Ｅ组大鼠系膜细胞仍有活跃的吞噬能力；Ｅ１组大鼠脾脏明显充血，白髓内出血，Ｅ２组大鼠脾白髓周围网状细胞增生，并出现广泛的铁沉积现象．发现一例肝细胞型肝癌。除白髓出血，增生的网状细胞取代某些趋近消失的滤泡外，尚可见局部胸腺依赖区明显再生．有２例肝纤维型间皮肉瘤。少数Ｅ组鼠胸腺内出血，淋巴结窦内和稳泡内出现红细胞．３次扑杀Ｃ组均正常。

Fifty-two adult male rats were divided into two groups. Thirty-five rats in the experimental group(E)were fed with corn contaminated with Afealoxin B1(1000μg·kg-1,while seventeen rats in the control greup (C) were fed with corn free of Afcantoxin. Rats killed at 8th,20 th and 4oth weeks.In some of E1,E2 group rats, the mesangium of kidney became thick with the proliferation of mescngial cells or mesangial matrix, In group E1 the earlist changes in the spleen were congestion of the red pulp and hemorrhage in white pulp. The reticular cells around the white pulps proliferated and hacmosiderin extensively deposited.In E3, besides the hemornhage of white pulp, the reticular cells proliferated almost subslituded the perishing fellides. In the periarteriolar sheath. T cell showed morve pronunced proliferation in E3 thanin E1 and E2,Changes in the kidney of E3 were the samre as thore of E1 and E2 groups, but the mombranous glomerulitis was mevre typical in appearance.It was found, two fibrosarcomas and hepatocytic cancer in three rats of E group,respectively.