期刊文献+

化学改性纤维素亲和膜色谱用作人血清白蛋白中杂质的去除 被引量:2

Impurity Removal of Human Serum Albumin by Affinity Membrane Chromatography with Chemically Modified Cellulose
下载PDF
导出
摘要 用环氧法对纤维素微孔滤膜进行了化学改性,并在经改性的膜上共价结合了活性红染料配基K-2BP,设计并制作了特殊的膜分离器,利用所制备的亲和膜分离器成功地去除了人血清白蛋白中的杂质。 Cellulose microfiltration membrane was chemically modified with epoxy method. Active red ligand, K2BP,was immobilized on the modified cellulose membrane.A special affinity membrane cartridge was designed and prepared.Impurities in human serum albumin were successfully removed bythe affinity membrane cartridge.
出处 《分析测试学报》 CAS CSCD 1995年第4期28-32,共5页 Journal of Instrumental Analysis
关键词 微孔滤膜 亲和膜分离器 人血清 白蛋白 Chemically modified cellulose,Active red dye ligand, Affinity membrane cartridge,Human serum albmin,Impurity removal.
  • 相关文献

参考文献1

共引文献8

同被引文献19

  • 1张亚辉,杨严俊.纤维素金属螯合亲和膜的制备及其对牛血清白蛋白的吸附研究[J].食品工业科技,2005,26(3):89-92. 被引量:3
  • 2张龙翔 张庭芳 李令媛.生化实验方法和技术[M].北京:人民教育出版社,1983.166-168.
  • 3Porath J, Carlsson J, Olsson I, et al. Metal chelate affinity chromatography:A new approach to protein fractionation[J].Nature, 1975, 258(5536) :598-599.
  • 4Porath J, Olin B. Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials:Serum protein affinities for gel-immobilized iron and nickel ions[J].Biochemistry, 1983. 22:1621-1630.
  • 5Keung Wing-ming, Parviz Azri. Structure and Function of Ovotransfreein[J]. The Journal of Biological Chemistry, 1982, 257 (3) : 1184- 1188.
  • 6Mashikhi S A AI,Shuryo Nakai. Sepatation of ovotransferrin from egg white by immobilized metal affinity chromatography[J]. Agric Biol Chem, 1997, 51(11) :2881-2887.
  • 7Porath J, Carlsson J, Olsson I, et al. Metal chelate affinity chromatography, a new approach to protein fractionation[ J]. Nature, 1975, 258(5536) : 598-599.
  • 8Wu C Y, Suen S Y, Chen S C, et al. Analysis of protein adsorption on regenerated cellulose-based immobilized copper ion affinity membranes [ J ]. J Chromatogr A, 2003, 996(1/2) : 53-70.
  • 9Anspach F B. Silica-based metal chelate affinity sorbents I. Preparation and characterization of iminodiacetic acid affinity sorbents prepared via different immobilization techniques[ J]. J Chromatogr, 1994, 672(1/2) :35-49.
  • 10郭为,商振华,于亿年,周康,周良模.大孔纤维素亲和膜在生化制剂纯化中的应用[J].中国药学杂志,1998,33(12):731-734. 被引量:6

引证文献2

二级引证文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部