摘要
本文通过ＦＡＣＳ（荧光激活细胞分选器）技术，利用单克隆抗体ＲＡ３－６Ｂ２抗Ｌｙ－５（Ｂ２２０）研究了Ｌｙ－５（Ｂ２２０）在新制备的小鼠脾脏Ｂ细胞上的表达，特别是用不同的丝裂原刺激而激活Ｂ细胞后Ｌｙ－５表达的变化，发现脾脏 Ｂ细胞形成两个主要细胞群，浮力密度较高的小Ｂ细胞高度表达 Ｌｙ－５（Ｌｙ－５ｈｉ），浮力密度较低的大Ｂ细胞则为Ｌｙ－５ｌｏｗ。用多克隆刺激因子ＬＰＳ（细菌脂多糖）刺激脾脏高浮力密度小Ｂ细胞，可诱导ｔｙ－５的表达降低。单独用膜交联剂抗－ＩｇＭ刺激，ｔｙ－５的表达仍保持较高状态，但当用抗－ＩｇＭ与白细胞介素－６一起刺激时，则诱导Ｌｙ－５的表达降低。这些结果表明，Ｂ细胞活化后Ｌｙ－５的表达降低，Ｌｙ－５ｌｏｗ可作为Ｂ细胞活化的一种标志。
The expression of Ly-5 (B220) in splenic B cell of mouse and the change of Ly-5 (B220) during B cell activation were studied by using FACS technique. The results showed that the splenic B cell form two populations. The smaller splenic B cell with high-density has the phenotype of Ly-5hi, and the larger population with low-density is of Ly-5low phenotype. Activation of resting B cells by LPS and anti-IgM induces differences in the level of Ly-5 expression. Stimulation of resting B cell of Ly-5hi. phenotype, but the latter remains high as the anti-IgM stimulation is used alone for. Additional stimulation of IL-6 following anti-IgM stimulation can decrease the level of Ly-5 expression. However, IL-1, IL-2, or IL-4, whether alone or in various combinations, can not induce the loss of Ly-5. Taking those together, we can conclude that the expression of Ly-5 goes down following B cell activation and Ly-5low may serve as an activation marker of B cell activation.
出处
《动物学报》
SCIE
CAS
CSCD
1995年第1期103-108,共6页
ACTA ZOOLOGICA SINICA
基金
中国自然科学基金资助
基金批文编号39100056
