卡尔曼滤波紫外光度法同时测定邻、间、对苯二酚

Simultaneous Determination of Catechol, Resorcinol and Hydroquinone with Kalman Filtering Ultraviolet Spectrophotometry

研究了苯二酚三种同分异构体水溶液的紫外吸收光谱，在ｐＨ３．６的缓冲溶液中，邻、间、对苯二酚的紫外吸收峰分别为２７５ｎｍ、２７３ｎｍ和２８８ｎｍ，三者重叠严重。选取在２４０ｕｍ～２９０ｎｍ范围内，每隔１ｎｍ测量一次吸光值共５１个点，采用卡尔曼滤波进行处理。可以获得较满意的结果。对１０个不同比例组成的标准混合液进行测定，邻、间、对苯二酚三者的平均回收率分别为９７．４％，１０１．７％和９８．０％，标准偏差分别为１．７２％，３．８６％和２．０４％。对模拟试样中加入标准的回收率均在９６．０％～１０３．０％之间。

The ultraviolet spectrophotometry based on Kalman filtering(KF) was applied to determine simultaneously three isomeric dihydroxybenzenes,catechol(1,2-dihydroxybenzene), resorcinol(1,3-dihydroxybenzene and hydroquinone(1,4-dihydroxybenzene) without preliminary separation.The ultraviolet absorption peaks of their aqueous solutions (pH=3.6) are 275nm,273nm and 288mp respectively.The average recoveries of catechol, resorcinol and hydroquinone in standard mixtures are 97.4%,101.7% and 98.0%,with standard deviations of 1.72%,3.86% and 2.04% (n=10),respectively.The proposed method has been used for the simultaneous determination of the thine isomers in simulating samples with satisfactory results.