三波长分光光度法研究Ⅲ．两测量波长法提高测定灵敏度

Study on Three-Wavelength Spectrophotometry Ⅲ.Sensitivity Enhancement by Dual Measuring Wavelengths Selection

本实验利用两测量波长法提高三波长分光光度法的灵敏度。选择合适的波长组合，消除两种共存组分的干扰，使△Ａ＝Ａλ１＋Ａλ２－ＫＡλ３只与待测组分的浓度有关，灵敏度一般均高于单波长法。以相对误差为选择波长组合的标准，编制了程序，用计算机选择合适的波长组合。验证结果令人满意。

The method of dual measuring wavelengths bas been proposed to enhance the sensitivity of three-wavelength spectrophotometry.To eliminate the mutual interference between two components,the measuring wavelengths are selected such that △A=Aλ1+Aλ2-KAλ3 is dependent only on the concentration of determination component.The relative error was taken as the criterion for wavelength combination selection, For this purpose, a computer programme was compiled.The testing results are satisfactory.