用层析法分离磷酸酯基化学修饰的二核苷酸的非对映异构体

Chromatographic Separation of Diastereomers of Dinucleotides Chemically Modified at Phosphate Backbone

本文报道用高效液相色谱法和高效薄层色谱法分离磷酸醌基化学修饰的二核苷酸二尿苷硼烷磷酸酯，二胸苷硫代磷酸酯，二（２＇－脱氧－２＇－氟）尿苷及胞苷硫代磷酸酯的非对映异构体，所得到的非对映异构体的ＲＦ值和＾３１Ｐ－ＮＭＲ化学移植之间的关系符合一般规律，即移动快的异构体有较高的位移植，移动慢的异构体有较的化学位移植。

The diastereomers of diuridine boranophosphate, dithymidine phosphorothiate, di (2'-deoxy-2'-fluoro) cytidine phosphorothioate, and di (2'-deoxy-2'-fluoro)uridine phos-phorothioate were separated by high-performance liquid chromatography or high-performance thin layer chromatography. The compounds with the higher RF values or shorter retention time have higher31P NMR chemical shifts and vice versa.