摘要
本文报道了一种显示鱼类染色体G-带的BrdU-BsG方法。采用肾细胞短期培养,收获前12小时加入BrdU,使终浓度为10μg/ml。制片经HCl、Ba(OH)_2处理,4×SSC温育。Giemsa染色,显示出白鲢的G-带。其带纹细致清晰,一个细胞的单倍染色体上显示带纹达200条以上,是目前已报道的鱼类多重带中带纹最多的,且反差明显,带纹有特征性,结果较稳定。根据实验结果初步建立了白鲢的G-带模式图。
A BrdU-BSG method is described for demonstration of G-banding in fish chromosomes.It involves culturing the kindney cells,treating cells with a final concentration of 10μg/ml BrdU for 12h before harvest.The air-dried slides are the treated in HC1 and Ba(OH)2,incubated in 4XSSC solution,and stained in Giemsa.Using this method,G-banding in the chromosomes of silver carp (Hypophihalmichihys moritrix) was observed with excellent results.There are at least 200 fine and distinct bands in the chromosome of a cell.An idiogram of G-banded karyotype of Hypophthalmichthys moritrix is constructed according to the experimental data.
基金
中国科学院科学基金
关键词
染色体
G带
模式图
鱼类
Hypophthalmichthys moritrix,G-banding,BrdU-BSG method,Idiogram
