类核沉降法分析247例癌症病人和正常人的DNA修复能力

Analysis of and Repair Capacity in 247 Cancer Patients and Normal Persons by Nucleoid Sedimentation Technique

应用新建立的类核沉降法,分析了108例食管癌、肺癌和卵巢癌患者以及139例正常人DNA修复能力。外周血淋巴细胞受2.5μJ/mm^2紫外线照射后,大多数正常人11小时完成DNA修复,而大多数癌患者17小时尚未能完成修复。

The nucleoid sedimentation technique was developed to analyze DNA repair capachy in 108 cancer patients (esophageal cancer 34,lung cancer 24 and ovarian cancer 50) and 139 normal persons.After exposing lymphocytes to UV in radiation at the dose of 2.5μ/mm2,the cells were incubated for different periods of time at 37℃ for repairing the damaged DNA.The nucleoid sedimentation distance which corresponds to DNA repair capacity was determined.It was found that most normal persons finished the process of DNA repair in II hours while the cancer patients could not do so even 17 hours ofter incubation.This study showed that decreased DNA repair capacity may be a component of the genetically determined sasceptibility to cancer.