同步荧光法同时测定苯甲酸和水杨酸的研究

Study on Simultaneous Determination of Benzoic Acid and Salicylic Acid by Synchronous Fluorimetry

根据苯甲酸与水杨酸在酸性条件下荧光光谱出现的差异，用同步荧光法同时测定其混合物中二组份。苯甲酸及水杨酸的检测限分别为０．２４μｇ／ｍＬ和０．２５μｇ／ｍＬ，相对标准偏差分别为１．３％和１．２％。方法用于商品杀菌防腐剂中苯甲酸与水杨酸的同时测定，结果满意。，Ｘｉｎｘｉａｎｇ，４５３００２）Ａｂｓｔｒａｃｔ：Ｂａｓｅｄｏｎｔｈｅｅｖｉｄｅｎｔｄｉｆｆｅｒｅｎｃｅ，ｏｆｆｌｕｏｒｅｓｅｅｎｃｅｓｐｅｔｒａｃｈａｒａｃｔｅｒｉｓｔｉｃｓｂｅｔｗｅｅｎｂｅｎｚｏｉｃａｃｉｄａｎｄｓａｌｉｃｙｌｉｃａｃｉｄｉｎ１．０ｍｏｌ／ＬａｑｕｅｏｕｓＨ２ＳＯ４ｓｏｌｕｔｉｏｎ，ａｎｅｗｍｅｔｈｏｄｈａｓｂｅｅｎｅｓｔａｂｌｉｓｈｅｄｆｏｒｓｉｍｕｌｔａｎｅｏｕｓｄｅｔｅｒｍｉｎａｔｉｏｎｏｆｂｅｎｚｏｉｃａｃｉｄａｎｄｓａｌｉｃｙｌｉｃａｃｉｄｉｎｍｉｘｔｕｒｅｓｂｙｓｙｎｃｈｒｏｎｏｕｓｆｌｏｕｒｉｍｅｔｒｙ．Ｔｈｅｄｅｔｅｃｔｉｏｎｌｉｍｉｔｓｏｆｂｅｎｚｏｉｃａｃｉｄａｎｄｓａｌｉｃｙｌｉｃａｃｉｄａｒｅ：０．２４μｇ／ｍＬａｎｄ０．２５μｇ／ｍＬ，ｒｅｓｐｅｃｔｉｖｅｌｙ．Ｔｈｅｒｅｌａｔｉｖｅｓｔａｎｄａｒｄｄｅｖｉ?

Based on the evident difference, of fluoreseence spetra characteristics between benzoic acid and salicylic acid in 1.0mol/L aqueous H2SO4 solution, a new method has been established for simultaneous determination of benzoic acid and salicylic acid in mixtures by synchronous flourimetry. The detection limits of benzoic acid and salicylic acid are: 0.24μg/mL and 0. 25μg/mL, respectively. The relative standard deviation are: 1.3% and 1.2%, respectively. The method has been successfully used to analyse benzoic acid and salicylic acid in commercial preser vative and bactericide simultaneously.