5，10，15，20－四（3－溴－4－磺酸苯基）卟啉光度法测定痕量锌的研究

Study on 5, 10, 15, 20-Tetra (3 - Bromo- 4-Sulphophenyl) Porphine for Spectrophotometric Determination of Trace Amounts of Zinc.

本文研究了新水溶性卟啉试剂５，１０，１５，２０－四（３－溴－４－磺酸苯基）卟啉［Ｔ（３－ＢｒＰ）ＰＳ４］与锌的显色反应。在ｐＨ４，锌与５，１０，１５，２０－四（３－溴－４－磺酸苯基）卟啉形成稳定的１：１配合物，其最大吸收波长位于４２２．０ｎｍ，摩尔吸光系数为３．９×１０５Ｌ·ｍｏｌ（－１）·ｃｍ（－１）。锌量在０～２．５μｇ／２５ｍＬ范围符合比尔定律。方法具有较高的灵敏度和较好的选择性，用于人发和大米中痕量锌的测定，结果与原子吸收法一致。

A new water soluble porphyrin, 5, 10, 15, 20-Tetra(3-bromo-4-sulphophenyl) Porphine [T(3-BrP)PS4] for spectrophotometric determination of trace amounts of zinc (Ⅱ) hasbeen Studied. The maximum absorption wavelengths of T(3-BrP)PS4 and its complex with zinc (Ⅱ) are 434. 8 urn and 422. 0 nm respectively at PH 4.0.The composition of the complex is 1: 1. The apparent molar absorptivity is 3.9×105 L·mol(-1)·cm(-1). Beer's law is obeyed in the range of 0～2. 5 μg/25 mL for zinc. The propaned method has been directly applied to determination of zinc in human hair and rice.The result obtained agrees well with that of the AAS.