摘要
从太平洋切奴克鲑鱼的垂体制备cDNA文库。按照鲑鱼泌乳素的部分蛋白质序列所提供的信息合成寡聚脱氧核苷酸探针。用探针筛查泌乳素基因,识别出一个阳性克隆PRL-10。该克隆的硷基序列已被测出。PRL-10的总长为1.1kb,编码了含有211个氨基酸组成的泌乳素前体,其中包括了编码23个氨基酸的信号肽序列和编码188个氨基酸的成熟泌乳素序列。
A cDNA library was prepared from pacific Chinook Salmon pituitaries.Salmon prolactin gene was screened using synthetic oligonucleotide probes based on partial protein sequence A positive clone (PRL-10) was identified and sequenced.It is a full size clone containing 1.1 kb and coding for a preprolactin of 211 amino acids.
基金
加拿大医学研究会
多伦多儿童医院基金
