~60Coγ射线诱发小鼠卵母细胞和骨髓细胞染色体畸变的量效关系比较

DOSE-RESPONSE RELATIONSHIP AND ITS COMPARI SONS OF ￣60Coγ-RAY INDUCED CHROMOSOME ABERRATIONS IN M Ⅱ OOCYTES AND BONE MARROW CELLS OF MICE

用不同剂量（０～３Ｇｙ）６０Ｃｏγ射线一次全身性照射雌性ＩＣＲ小鼠，观察ＭⅡ（第二次减数分裂中期）卵母细胞和骨髓细胞染色体损伤效应，用ＷＨＯ推荐的４个模式进行剂量效应曲线的拟合，选出最优的拟合模式，并对ＭⅡ卵母细胞和骨髓细胞的量效关系式进行了比较。结果表明，ＭⅡ卵母细胞和骨髓细胞均为辐射的敏感指标。骨髓细胞的辐射敏感性高于ＭⅡ卵母细胞。

Chromosome aberrations indUced by various 60Co γ-ray doses(0-3Gy) were examined in secondary oocytes and bone marrow cells of ICRmice. The chromosome aberration rates of the two cell lines were both significantly higher in γ-irradiated groups than that in control ones. Using four modelsrecommended by WHO, dose-response calibration curves were established and bettermatches were selected= they were Y = 21. 1057 D0.7958,Y = 1. 6502 + 15 .9986 D +3.8533 D2 for aberrant cell and total chromosome aberration rates(% ) of oocytes,respectively. And Y = 29. 8031 D0.5186, Y= 3. 1905 + 27. 1214 D + 8 .5663 D2 for aberrant cell and total chromosome aberration rates(%) of bone marrow cells, respectively. A comparison of radiosensitivity between ME oocytes and bone marrowcells were also made, the results showed that both Ma oocytes and bone marrowcells were sensitive, the bone marrow cell radiosensitivity was higher than that ofM Ⅱ oocytes by 2 .2 times.