催化动力学法测定人体血清中痕量铜的研究

Determination of Trace Copper in Serum by Catalytic Kinetic Spectrophotometry

研究Ｃｕ^（２＋）在稀氨水介质中催化Ｈ_２Ｏ_２氧化苯基荧光酮褪色反应及影响反应速率的动力学因素。据此建立催化动力学法测定痕量Ｃｕ^（２＋）的新方法，其灵敏度为５．０×１０^（－１１）ｍｏｌ／Ｌ，测定范围为（０～２．５）×１０^（－７）ｍｏｌ／Ｌ。操作简便、快速。该法可用于人体血请中痕量铜的测定。

Copper(Ⅱ)in NH3·H2O can catalyze a new discolouring reaction in which hydrogen peroxide oxidizes phenylflourone,and its kinetic condition is proposed.A new catalytic spectrophotometric method of determining trace copper(Ⅱ)was obtained.The sensitivity of the method is 5.0×10-11mol/L,with the range of determination being(0～25)×10-8 mol/L.The method has been used to determine trace copper(Ⅱ)in serum samples with satisfactory results.