骨和软骨肉瘤中骨形成蛋白的免疫组织化学定位

Immunohistochemical Localization of Bone Morphogenetic Protein in Osteosarcoma and Chondrosarcoma

用抗牛骨形成蛋白单克隆抗体(bBMP-McAb)研究了骨形成蛋白(BMP)在骨肉瘤及软骨肉瘤中的细胞定位。ABC法染色阳性12/14(骨肉瘤);4/4(软骨肉瘤)结果显示,BMP主要分布于骨和软骨肉瘤的肿瘤细胞胞浆及部分肿瘤性骨组织中,从而证实骨、软骨肉瘤都含有丰富的BMP。用bBMP-McAb染色不仅可以将骨肉瘤与纤维肉瘤等非骨源性肿瘤加以鉴别,而且可以根据BMP在肿瘤中的分布和含量,结合患者的临床情况可望对骨肉瘤进一步分类,为临床治疗提供依据。此外,对于软骨肉瘤内含有丰富的BMP的首次发现,揭示BMP可能与其它的骨疾患有密切的关系。

The monoclonal antibody against bovine bone morphogenetic protein (bBMP-McAb) was first used for demonstration of bone morphogenetic protein (BMP) in 14 patients with osteosarcoma and four cases with chondrosarcoma. Using avidin-biotin complex method (ABC), we demonstrated that BMP mainly existedin the tumor cell plasma and some tumor-like bone tissues with positive staining in-12 out of 14 osteosarcoma patients and in all the four cases of chonbrosarcoma. Using this staining method, we can not only differentiate osteosarcoma from fidrosarcoma (all are negative) and other non-osteogenic tumors, but also further classify osteosarcoma according to the BMP content and distribution and the patients clinical treatment. In addition, the findingsof rich BMP in chondrosarcoma indicates that BMP may be closely related with other bone diseases.