大鼠异种心脏移植实验研究

Experimental Discordant Heart Xenografts in the Rat

研究通过建立豚鼠－大鼠异种心脏移植动物模型，采用血浆置换方法降低血浆天然抗体含量，并结合脾切除及应用免疫抑制剂──环磷酰胺抑制抗体的产生，观察移植物存活时间。实验结果表明：血浆置换法可明显减少抗体的含量，血浆１ｇＭ和１ｇＧ分别从置换前９３６．０ｍｇ／Ｌ和２４２４．３ｍｇＬ降到１６７ｍｇ／Ｌ和６９３．３ｍｇ／Ｌ，与置换前血浆抗体水平相比有显著性差异。对照组移植存活时间为１３．２ｍｉｎ，而血浆置换组平均存活时间为５３７．０ｍｉｎ；同时使用环磷酰胺和脾切除组可显著地延长异种移植物超急性排斥反应的发生，平均存活时间为６７０．０ｍｉｎ。

The removal and subsequent suppression of natural antibodies is thought to be central to the potential success of discordant xenografting,The purpose of this study was to analyse the effect of plasmapheresis,cyclophosphamid and splenectomy on the depletion and return of natural antibodies and on the survival time of the heterotopic guinea pig-to-rat cardiac xenotransplantation. In the control group, the mean survival time was 13. 2 min.When plasmapheresis was performed immediately prior to transplantation(Group 2),the graft survival time was 537. 0 min.The combination of plasmapheresis with cyclophosphamid and splenectomuy demonstrated a further prolongation of graft survival(760. 0 min).The results of this study indicate that plasmapheresis coupled with other therapies may be a useful adjuvant in xenogeneis transplantation.