头孢氨苄降解产物荧光性质的研究及分析应用

Studies on Fluorescent Ptoperty of Degradation Product of Cephalexin and Its Analytical Application

详细研究了头孢氨苄在酸、碱介质中的降解反应，建立了酸、碱降解的荧光分析法．线性范围是０．５０～１００ｎｇ／ｍＬ（酸降解）和２．０～７０ｎｇ／ｍＬ（碱降解）．从精密度和准确度看，酸降解优于碱降解的荧光分析．将酸降解荧光分析法应用于血清和尿样中头孢氨苄的测定，结果良好．

In this paper, the degradation reactions of cephalexin in acid and alkali media are deseribed in detail,and the corresponding fluorometric methods of cephalexin are presented by producing its stable fluorescent products. The linear ranges are 0. 50-100 ng/mL (acid degradation) and 2.0─70 ng/mL (alkali degradation), respectively. The fluorometry with acid degradation has more advantages of analytical precission and is more accuracy than that with alkali degradation,which has been used for the determination of trace cephalexin in human serum and urine with satisfactory results.