疏水界面上标准蛋白质吸附等温线的研究

Studies on Adsorption Isotherms of Proteins on Hydrophobic Surface

测定了几种具有代表性的标准蛋白质在疏水色谱填料上的吸附等温线。除溶菌酶和牛血清白蛋白的吸附近似呈线性外，细胞色素－ｃ、肌红蛋白、胰岛素、α－淀粉酶和卵清蛋白均呈凸型吸附。用Ｌａｎｇｍｕｉｒ、计量置换吸附模型（ＳＤＭ－Ａ）、ＢＥＴ和Ｊｏｖａｎｏｖｉｃ吸附模型对这些蛋白的吸附等温线拟合后发现，ＳＤＭ－Ａ关系式能够良好描述这７种蛋白的吸附，Ｌａｎｇｍｕｉｒ关系仅能较好地描述后５种蛋白质的吸附。而Ｊｏｖａｎｏｖｉｃ和ＢＥＴ关系式对上述两类蛋白质吸附的描述欠妥。实验发现对于不同盐浓度条件下卵清蛋白的吸附，用ＳＤＭ－Ａ和Ｌａｎｇｍｕｉｒ公式拟合所得的参数与盐浓度之间呈现出一定的规律性。

Adsorption isotherms of proteins on hydrophobic surface were measured by frontal chromatography.It was found the adsorption isotherms of lysozyme and bovine serum albumin to be linear and those of cytochrome-c,myoglobin,insulin,α-amylase,and ovalbumin to be convex.With the comparison among stoichiometric displacement model of adsorption(SDM-A),the Langmuir model,the BET equation,and the Jovanovic equation,the first one was found to fit two kinds of adsorption isotherms mentioned above well,the second one could only be used to describe the latter well,while the last two equations could not be used to describe anyone of them well.For ovalbumin,the linear parameters of the SDM-A and the Langmuir model were found to have certain regularity with the changes in the concentrations of ammonium sulfacte.