完全重叠色谱峰的二维微分色谱解析算法

Resolution of Completely Overlapping Chromatographic Peaks by First Differentiation of Two-Way Data

利用完全重叠的色谱峰中同时消失的两组份的浓度变化速率差异，通过二维数据微分色谱法获取被掩盖组份的近似光谱，以解析完全重叠的色谱体系。将该法应用于维生素Ｃ和菸酸两组份完全重叠的二维色谱之分辨，结果令人满意，分辨光谱与标准光谱重合甚好。

A new approach to resolve completely overlapping chromatographic peaks is developed in this paper.Taking the advantage of different disappearing rates of two components,the method can obtain the approximate spectrum of embedded component by first differentation of two-way data.Applying the method to resolving the two-way chromatograms with two overlapping peaks of ascorbic acid and nicotinic acid,the estimated spectra accord well with the pure ones.The result is satisfactory.