冠醚修饰的固体支撑双层类脂膜的形成及性能研究

The Formation and Performance of Solid Supported Bilayer Lipid Membranes Modified with Crown Ethers

用饱和了胆固醇和修饰用冠醚的角鲨烷／氯仿溶液作成膜液，制备了冠醚修饰的固体支撑双层类脂分子膜（ｓ－ＢＬＭｓ）．重点考察了成膜物种及技术对膜稳定性及电特征的影响．其膜电势随接触水相中［Ｋ~＋］的变化呈现Ｎｅｒｎｓｔ响应，线性范围１０^（－４）～１０^（－１）ｍｏｌ／Ｌ．

Self-assembly solid supported bilayer lipid membranes(S-BLMs)modified with crown ethers were formed on metalic wire.The stability and the electrochemical characteristics of the s-BLMs depended on the variety of metalic wire,organic solvent,lipids and molecular structures of crown ethers. The membrane potentials of the s-BLMs prepared with stainless steel wire in mixed solution of squalane saturated cholesterol and crown ether in CHCl3 were determined.The cavity size and negative electricity of the ether ring of crown ethe,r molecules are favorable to the selective complexation with metal cations(M+,e.g. Li+ Na+,K+) in the aqueous solution. The change of[M+](10-4-10-1 mol/L)would result in a Nernst corresponding in the membrane potential.