硫堇－甲苯二异氰酸酯衍生物修饰的玻碳基乙醇传感器的研究

A Reagentless Alcohol Sensor Based on Thionine-TDIModified Glassy Carbon Electrode

硫堇－甲苯二异氰酸酯衍生物修饰的玻碳基乙醇传感器的研究俞爱民，韩吉林，杨可盛，陈洪渊（南京大学化学系，南京，２１００９３）关键词硫堇－ＴＤＩ衍生物，ＮＡＤ~＋，ＡＤＨ，乙醇传感器经典的测定乙醇含量的方法有气相色谱法［１］、分光光度法［２］．近年来，随?..

Alcohol dehydrogenase(ADH) and the oxidized form of nicotinamide adenine dinucleotide(NAD+) were cross-linked directly in aid of the reaction of thionine and toluene diisocyanate(TDI) on the surface of glassy carbon electrode to yield a reagentless alcohol sensor. The NAD+ was converted to NADH by oxidation of alcohol catalyzed by ADH and the NADH formed was oxidized electrochemically to the original NAD+ by thionine-TDI mediator, thus giving the catalytic current for target analysis. The peak current was linearly related to the concentration of alcohol in the range of 1-15 mmol/L. The influence of various experimental conditions was also explored for optimum analytical performance.The sensor remains relatively stable for about 15 days.