摘要
硫堇-甲苯二异氰酸酯衍生物修饰的玻碳基乙醇传感器的研究俞爱民,韩吉林,杨可盛,陈洪渊(南京大学化学系,南京,210093)关键词硫堇-TDI衍生物,NAD~+,ADH,乙醇传感器经典的测定乙醇含量的方法有气相色谱法[1]、分光光度法[2].近年来,随?..
Alcohol dehydrogenase(ADH) and the oxidized form of nicotinamide adenine dinucleotide(NAD+) were cross-linked directly in aid of the reaction of thionine and toluene diisocyanate(TDI) on the surface of glassy carbon electrode to yield a reagentless alcohol sensor. The NAD+ was converted to NADH by oxidation of alcohol catalyzed by ADH and the NADH formed was oxidized electrochemically to the original NAD+ by thionine-TDI mediator, thus giving the catalytic current for target analysis. The peak current was linearly related to the concentration of alcohol in the range of 1-15 mmol/L. The influence of various experimental conditions was also explored for optimum analytical performance.The sensor remains relatively stable for about 15 days.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1995年第8期1204-1206,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
中国科学院长春应用化学研究所电分析化学开放实验室基金