分离蛋白质的高效离子交换色谱填料的研究（Ⅳ）──强阴离子交换色谱填料的合成及应用

Studies on the New Packings of High Performance Ion Exchange Chromatography for the Separation of Proteins(Ⅳ)──The Synthesis and Application of the Packings of the Strong Anion Exchange Chromatography

含环氧基的硅胶与二乙胺或聚乙烯亚胺反应，其产物再与碘甲烷或氯化苄反应，得到含季铵离子的强阴离子交换色谱填料。该填料可用于蛋白质和生物工程产品（如基因重组人干扰素γ、粒细胞－巨噬细胞集落刺激因子和人绒毛膜促性腺素等）的分离纯化。其中，环氧基硅胶与二乙胺加成后再用氯化苄季铵化的产物是较好的强阴离子交换色谱填料。

The silica with epoxy group reacted with diethylamine or polyethyleneimine, then the product reacted with methyl iodide or benzyl chloride and yielded the packings of strong anion exchange chromatography with quaternary ammonium cation. These packings can be used for separating proteins and biotechnology product, such as IFN-γ,GM-CSF and HCG.