辅酶Ⅰ在β－萘醌磺酸根修饰金带电极上的电化学还原

The Electrochemistry of Nicotinamide-adenine Dinucleotide at a β-Naphthochinonsulfonicum Modified Gold Band Electrode

研制了聚毗咯／ＮＱＳ修饰金带电极，讨论了ＮＱＳ／ＰＰｙ的电聚合过程及实验条件对金电极上吡咯聚合物膜性质的影响；用成核及生长理论解释了阴离子ＮＱＳ对电聚合过程计时电流曲线的影响，优化了ＮＱＳ／ＰＰｙ／Ａｕ电极的制备条件．该修饰电极稳定性好，对辅酶Ⅰ在金电极上的还原有催化作用．在ｐＨ＝７．０时催化电流在４．０×１０￣（－５）～１．５×１０￣（－３）ｍｏｌ／Ｌ范围内与ＮＡＤ￣＋ 浓度有线性关系，探讨了电催化作用的机制．

The preparation of β-naphthochinonsulfon-icum(NQS) modified gold band electrode was studied.The electropolymerization process of NQS/PPy and the effects of experimental conditions on the behaviour of polymer membrane were discussed. The effect of anionic NOS on chronoamperometric curves in electropolymerization process was explained with the nucleation and growth theory. The modified electrode can catalyze the electrochemical reduction of NAD+ at a gold electrode. At pH 7.0,the catalytic peak current is proportional to the concentration of NAD+ in the range of 4.0× 10(-3) to 1. 5×10(-3) mol/L. The electrocatalvtical mechanism was also discussed.