分离蛋白质的高效离子交换色谱填料的研究（Ⅲ）──膦酸基阳离子交换色谱填料的性能

Studies on the New Packings of High Performance Ion Exchange Chromatography for the Separation of Proteins(Ⅲ)──The Characteristics or the Packings of Cation Exchange Chromatography with Phospbono Group

分离蛋白质的高效离子交换色谱填料的研究（Ⅲ）──膦酸基阳离子交换色谱填料的性能常建华（西北大学化学系，西安，７１００６９）关键词填料，高效阳离子交换色谱，性能，蛋白质以磷羟基为离子交换基团的色谱填料是一类中强阳离子交换色谱填料，以前报道的此类填料是使...

The packings of cation exchange chromatography with phosphono group and matrix of silica for separation of proteins have been synthsised, and the relationship between their structure and characteristics has been studied.This kind of packings of cation exchange chromatography with medium intensity has a high recovery of the bioactivity of proteins,better separation function and wider applied scope than those of the weak exchange chromatography in the previous papers.The protein load capacity of these packings is about 20 mg/mL for a sample of Lysozyme by the dynamic load capacity determination method.The use scope of pH is 3.5 to 8. The bioactivity recovery of Lysozyme is 96.7%.It is noteworthy that the hydrophobicity of ligand has an effect on the retention of proteins in IEC. With the increase of the ligand hydrophobicity, the retention of proteins increases. On the other hand, the change of the salt concentration in the mobile phase can change the hydrophobic action between proteins and packings, therefore change the retention of proteins. When two proteins have a great hydrophobic difference and a near retention in the IEC, maybe the change of the salt concentration can get a good separation result.