摘要
Trp(色氨酸)与Tyr(酪氨酸)二阶微分光谱能被用来在线检测RP-HPLC(反相高效液相色谱)中含芳香族氨基酸的肽段,这些特征光谱是通过二极管矩阵检测器(DAD)得到的,用这种方法分析人表皮生长因子(hEGF)的胰蛋白酶酶解图谱,能鉴别出含Trp的C-端肽。
The characteristic second-order derivitive spectra of tryptophan and tyrosine are described and used toidentify the peptides containing tyrosine and tryptophan residues during their elution from reversed-phaseHPLC. Spectral data were acquired with a scanning diode array detector.The method allows the non-destructive identification of tryptophan residues in tryptic maps of hEGF and enables the selection andisolation of C-terminal of hEGF.
出处
《高技术通讯》
CAS
CSCD
1995年第5期7-10,共4页
Chinese High Technology Letters
基金
863计划资助项目