在双水相系统中生物转化丙烯腈为丙烯酰胺的研究

Bioconversion of Acrylonitrile to Acrylamide in Aqueous Two-Phase System

本文研究了以产腈水合酶的恶臭假单胞菌JP-1,在聚乙二醇/磷酸盐双水相系统中转化丙烯腈为丙烯酰胺的条件.双水相系统的组成为:聚乙二醇6000浓度0.05g/ml,磷酸氢二钾浓度0.20g/ml,丙烯腈浓度0.30mol/L,含酶细胞浓度0.10g/ml,pH9.0.25℃时转化率最高;pH10.0时酶最稳定;温度越低,酶的稳定性越好.还研究了在反应过程中间歇补充丙烯腈生成丙烯酰胺,以及从反应液中提纯丙烯酰胺的方法.

Acrylamide was produced from acrylonitrile in aqueous two-phase system by Pseudomonas putida JP-1 cells containing nitrile hydratase. The aque-ous two-phase system consisted of polyethylene glycol 6000(0.05g/ml), K2HPO4 ·3H2O(0.20g/ml), acrylonitrile (0.30mol/L), wet cells (0.l0g/ml), pH9.0. The optimum temperature 25℃ for the enzymatic conversion was determined. At pH 10.0, nitrile hydratase in Pseudomonas putida JP-1 cells was stablest. The lower the temperature, the better the heat stability of nitrile hydratase in cells. During the bioconversion course, acrylonitrile was added at certain time intervals to produce acrylamide, then the acrylamide was purified.