抗结肠癌和抗癌胚抗原的单克隆抗体识别大肠良恶性病变的对比研究

Comparison study on monoclonal antibody against colo -rectal carcinoma and carcinoma embryo antigens in de -tection of colnic malignant lesions

应用抗癌胚抗原(CEA)和抗结肠癌单克隆抗体(MC_3)对大肠癌以及腺瘤等良性病变的结肠粘膜进行了免疫组织化学(PAP法)研究.结果CEA在大肠癌和重度不典型增生的腺瘤组织中阳性率为100％,在正常结肠组织和其它非腺瘤的良性病变组织中良性率为33.33—60.00％,在轻、中度不典型增生腺瘤组织中分别为74.58％和84.62％.MC_3相应抗原在大肠癌中阳性率为100％,在轻、中、重度不典型增生腺瘤组织中阳性率分别为44.07％、69.23％、100％,在正常结肠粘膜及良性非腺瘤性病变组织中阳性率为0—22.22％.以上结果表明MC_3和抗CEA两种单克隆抗体在识别恶性肿瘤方面的敏感性均佳,但MC_3特异性远优于抗CEA单克隆抗体.

To evaluate monoclonal antibody against human colorectal caricnoma ( MC3 ) in detection of colorectal carcinoma and compare it with anti-CEA monoclonal antibody, we examined histochemically ( with PAP method ) the reactivity of the MC3 and anti-CEA to the colorectal tissues of normals, carcinomas and mucosa adjacent to carcinoma, adenomas, benige non-adenoma lesions (chronic colitis, inflammatory polypses, juvenile polypses, hyperplastic polypses and P-J polypses ) . The MC3 could stain all the 43 carcinomas. The staining positive rate in normals, mucosa adjacent to carcinoma and benign non-adeplasia were 44.07% ,69.23% and 100% respectly. These results show that MC3 can differentiate colorectal carcinoma from normal mucosa, benign non-adenoma lesions and adenoma with low degree of dyspla-sia. It might be helpful in detection of malignant potential of precancerous lesions. Anti-CEA were able to stain all carcinoma and increase staining positive rate along with the degree of dysplasia ( 74.58%, 84.62%, 100% for mild, moderate, severe respectly)too.But the positive rate in normals,mucosa adjacent to carcinoma and benign non-adenoma lesions were 33.33%-60.00%. This leads us to conclude that MC3 is better than anti-CEA in specificity.