免疫酶染色试验诊断广州管圆线虫感染鼠的研究Ⅱ─—感染度和感染持续时间对抗体生成的影响

Diagnosis of Angiostrongylus cantonensis Infection by IEST in Rats.Test Ⅱ:Effect of intensity and Duration of Infection on the production of Specific Antibody

用ＩＥＳＴ法研究感染度与感染持续时间对广州管圆线虫感染鼠抗体生成的影响。感染２０，５０和１００条第３期幼虫的三组实验鼠，８星期后用ＩＥＳＴ法检测血清中特异性抗体，ＧＭＲＴ分别是６５．０，２９８．６和３Ｇ７．６，显示抗体滴度随感染度增大而递增趋势，而对照级则呈阴性。２０只感染５０条幼虫的实验鼠分别于感染前和感染后３，８和１３星期检测，ＧＭＲＴ分别是１０５，５，２９８，６和１９０．３，滴度高峰在感染后８星期。实验表明，特异性抗体水平与感染度及感染持续时间密切相关。

The influences of intensity and duration of infection of Angiostrongylus cantonensis onthe production of specific antibody were studied using IEST technique.8 Weeks after infect ion with20,50 and 100 third一stage larvae,their GhIRT were 65.0,298.6 and 357.6,respectively,showingthat the specific antibody increascd in accordnnce with the intcnsity of infection,In the group of 20rats infected with 50 third一stage larvae, the GMRT was ncgative before infection,and 105.5,298. 6 and 190.3 at3,8 and 13 weeks after infection restoectivelly,with the peak at the 8th weck after in-fection,The results suggest that the levels of specific antibody are closely related to the intensity andduration of infection.