狂犬病毒aG株糖蛋白膜外区基因克隆和序列分析

Molecular Cloning and seguencing of the Glycoprotein Ectodomain Gene of a G Strain of Rabies Virus

本文应用聚合酶链反应（ＰＣＲ）技术获得了狂犬病毒ａｃ株（ＲＶａＧ）糖蛋白膜外区基因克隆并进行了序列分析。测出其核苷酸序列与其他株相应区序列的同源性在９０．１２％（ＣＶＳ／ａＧ）到９３．０３％（ＳＡＤＢ１９／ａＧ）之间，推导氨基酸序列的同源性在９１．４１％（ＣＶＳ／ａＧ）到９３％，２３％（ＰＶ／ａＧ）之间，经分析发现成熟肽推导氨基酸第１８２位和１８３位的替代，导致了乙酰胆碱受体（ＡＣｈＲ）结合序列的构象改变，可能是造成ＲＶａＧ毒力减弱的分子基础。

we have cloned and sequenced the glycoprotein ectodomain gene of aG strain of rabies virus(RVaG)by using polymerasc chain reaction(PCR)method. Compering its nucleotide scquences with the corresponding sequences of other strains revealed that the gornologies of the nucleotide se-quences were between 90.12%(CVS/aG)and 93.03% (SADB19./aG)and the homologies of the de-duced amino acid sequences between 991.4%(CVS/aG)and 93.23%(PV/aG).By comparing and analyzing,we bdteved that the replacements on 182 of the deduced amino acid in the matured peptide of the glycoprotein had resulted in the configuration change of the sequence linking to acetyl-choline receptor (AChR),wbich could have reduced the virulence of RVaG.