蛋白激酶C在鼻咽癌细胞生长中的作用

EFFECT OF PROTEIN KINASE C ON NASOPHAPHARYNGEAL CARCINOMA CELL LINE GROWTH

通过耗竭蛋白激酶Ｃ（ＰＫＣ），用ＰＫＣ抑制剂和激活剂等方法，观察ＰＫＣ在鼻咽癌细胞系ＣＮＥ－２Ｚ生长中的作用。发现：随ＴＰＡ浓度增大，细胞ＰＫＣ含量降低，生长指数也逐渐减少；作用于ＰＫＣ蛋白催化区的ＰＫＣ抑制剂Ｓｔａｕｒｏｓｐｏｒｉｎｅ（ＳＴ）与作用于调节区的抑制剂Ｓｐｈｉｎｇｏｓｉｎｅ（ＳＳ）均抑制ＣＮＥ－２Ｚ细胞的生长，最低有效浓度分别为２×１０－７ｍｏｌ／Ｌ与１０－５ｍｏｌ／Ｌ，随浓度增大而作用增强；ＰＫＣ刺激剂ＯＡＧ（０．１～１００μｇ／ｍｌ）单独作用时对ＣＮＥ－２Ｚ的生长无明显影响，但４μｇ／ｍｌ可基本阻断ＳＴ或ＳＳ最低有效浓度的抑制效应。提示：ＰＫＣ在鼻咽癌细胞ＣＮＥ－２Ｚ生长中起重要作用，阻断ＰＫＣ的作用将为鼻咽癌的防治提供新的途径。

The role of protein kinase C(PKC)in the growth of nasopharyngeal carcinoma (NPC)epithelial cell line(CNE-2Z)was further studied by consuming PKC,and using inhibitors or stimulator.12-o-tetra-decanoylphorbol-13-acetate(TPA)(10-5mol/L) could partly consume PKC to 47.6%of control and cellular growth was inhibited by 40.2%of the control.Staurosporine(ST),a potent inhibitor acting on catalytic domain of PKC,and sphingosine(SS) ,a potent inhibitor acting on modulating domain,both could inhibit the growth of CNE-2Z.The lowest effective concentrations of ST and SS were 2×10-7mol/L and 10-5mo1/L respectively.Although OAG,a stimulator of PKC,could not make marked effect on cellular growth at concenttraion from 0.1μg/ml to 100μg/ml,4μg/ml basically blocked the inhibition effect of ST(2×10-7mol/L)and SS(10-5mol/L).Authors'findings give a strong suggestion that PKC plays an important role in growth of CNE-2Z cells,so blocking the action of PKC may be a new pathway to prevent and cure NPC.