鸡传染性喉气管炎的研究　Ⅰ．应用间接ELISA法检测鸡传染性喉气管炎病毒抗体

Study on Avian infectious Laryngotracheitis Ⅰ. Using indirect ELISA for the Detection of Antibodies to Avian Infectious Laryngotracheitis Virus in Chicken Serum

采用原代鸡胚肾（ＣＥＫ）细胞增殖鸡传染性喉气管炎病毒（ＩＬＴＶ），并用ＰＥＧ－２００００粗提病毒蛋白抗原，建立了用来检测抗ＩＬＴＶ抗体的间接酶联免疫吸附试验。应用该法检测从广西９个大、中型鸡场采集的２３９份未免疫鸡血清样品，结果血清样品阳性率为４５．１９％，各个鸡场均为阳性场（１００％），首次证实广西存在ＴＬＴＶ抗体阳性鸡群．

This experiment multiplied avian infectious laryngotracheitis virus using primary chicken embryo kidney cell and virus-protein was crudely extracted with PEG--20000.An indirect enzyme-linked immunosorbent assay to detect avian infectious laryngotracheitis virus antibodies was established. 239 serum specimens from 9 farms in Guangxi were detected by indirect ELISA. The results demonstrated that the positive rate of serum specimens is 45. 19%, 100% flocks were positive. It is the first report that there exist serological positive avian infectious laryngotracheitis flocks in Guangxi.