小儿肾病综合征红细胞膜流动性的观察

OBSERVATION OF ERYTHROCYTE MEMERANE FLUIDITY IN CHILDREN WITH NEPHROTIC SYNDKOME

以１，６－二苯基－１，３，５－己三烯为荧光探剂，用荧光偏振法测定了２０例肾病综合征（ＮＳ）患儿的红细胞膜流动性，２０名健康儿童为正常对照组。结果表明ＮＳ患儿红细胞膜流动性明显低于正常对照组。患儿红细胞膜由高血脂的血浆中获得了较多的胆固醇是其流动性降低的原因。揭示红细胞膜流动性降低造成红细胞流变学异常，可能在ＮＳ血栓形成中起一定的作用。

The fluidity of the erythrocyte membrane in 20 children with nephroticsyndrome (NS) and 20 normal children was deter mined by the fluorescence polarization tech-nique usingl,6-diphenyl-1,3,5-hexatriene(DPH) as the fluorescence probe. The resultsshowed that the fluidity of the erythrocyte membmne in the children with NS was lowersigniflcantly than the normal controls. The reason of such lower fluidity was that theerythrocyte membrane of NS patients obtained more cholesterol than normal form theirhyperlipemic plasma. The result suggests that the lower fluidity of the erythrocyte membranecan produce dynamic abnormality of the erythrocytes,thus,playing an important role in themechanism of thrombosis in NS children.