短期培养和长期培养的自体LAK细胞的制备和应用研究

PREPARATION AND USAGE OF SHORT TERMAND LONG TERM CULTURED AUTOLO-GOUS LAK CELLS

用密度梯度离心法分离单个核细胞，在体外经短期（３－５ｄ）和长期培养（７－１５ｄ）后将自体ＬＡＫ细胞回输给病人。结果显示：长期培养ＬＡＫ细胞的圹增细胞数明显多于短期培养ＬＡＫ细胞扩增数；两者在体外均能杀伤人髓样白血病Ｋ５６２细胞，前者的平均杀伤活性为３９．２４±２．８１％，后者的平均杀伤活性为３５．９９±１．３１％，病人经自体ＬＡＫ细胞治疗后，其症状和体征均有所改善。

Mononuclear leukocytes were obtained by density gradient seperation, and were cultured for a short term(3-5 days) and a long term(7-15 days )in vitro. The rate of proliferation of the long term cultured autologous LAK cells was higher than that of the short term cultured autologous LAK cells (P<0.005). The cytotoxicity of the short term cultured autologous LAK cells and the cytotoxicity of the long term cultured autologous LAK cells upon human erythroleukemic tumor line k562was 39.24±2.81% and 35.99±1.31% respectively (P<0.05). The signs and symptoms of the patients improved after the treatment with autologous LAK cells.