菊芋悬浮细胞系的建立及其导管分子的体外分化

Suspension Cultures and Tracheary Elements Differentiation in vitro in Helianthus tuberosus L.

利用菊芋地下块茎作外植体,在附加1.2mg·L^(-1)2,4-D 的 MS 培养基上诱导愈伤组织形成,经同样 MS 液体培养基培养形成悬浮系;菊芋悬浮细胞转入含5.0mg·L^(-1)的6-BAP、1.0mg·L^(-1)2,4-D 的 MS 液体培养基内.经9d 培养可使约30%～50%悬浮细胞转化为具次生壁条纹的导管分子。

The stem tubers of Jerusalem artichoke(Helianthus tuberosus L.)were cultured on MS media with 1.2mg·L^(-1)2.4-D.Callus were induced and suspension cultures were formed.About 30%～ 50% cells differentiated into tracheary elements on MS media with 1.0mg·L^(-1)2.4-D and 5.0mg·L^(-1)6- BAP for 9 days.Elongated cells generated banded wall patterns,while spherical cells generated webbed wall patterns.