罗非鱼肝细胞作为环境污染生物监测指标的初步研究

A PRELIMINARY STUDY ON T.MOSSABICA HEPATOCYTE AS A BIOLOGICAL MONITORING INDEX OF THE EVIRONMENTAL POLLUTION

应用细胞生物学技术，研究了河北白洋淀及江西乐安江水样对罗非鱼原代肝细胞的影响。结果表明，白洋淀府河的４个采样点（望亭、际头、大桥、王家寨）水样对罗非鱼肝细胞具有生物毒性。入淀后另２个水样（刘庄、枣林庄）则基本无影响，在乐安江所采集的３个水样中，接渡样点样品对罗非鱼肝细胞毒性最大，沽口、虎山次之。

Cytological technology based on in-vitro primary culture of T.mossabica hepatocyte was developed and applied for screening the toxicities of environmental water samples from two typical regions regions.The resutts show that four water samples from up-stream of Fuhe river,Baiyangdian lake area,had significant cyto-toxicity while another two samples from down-stream had no significant influence on T.mossabica hepatocyte.Among three water samples from Lean river,Jiangxi provinee,the observed cyto-toxicites in toxic screening was comparable to P.phosphoreum bioassay.