平板免疫溶血试验快速检测大肠杆菌不耐热肠毒素

DETECTION OF HEAT─LABILE ENTEROTOXIN(LT)OF ENTEROTOXIGENIC E .COLI FROM VARIOUS ORIGINS IN PLATE IMMUNE HAEMOTOCYTOLYSIS ASSAY

琼脂糖为介质，进行固相平板免疫溶血试验测定毒素源性大肠杆菌不耐热肠毒素（ＬＴ），同时与ＥＬＩＳＡ，兔肠结扎试验测定ＬＴ进行了比较。结果表明，平板免疫溶血试验特异性强，标准的ＥＴＥＣ发生溶血，非ＥＴＥＣ不发生溶血，灵敏度与ＥＬＩＳＡ相似，比肠结扎试验敏感８倍左右；对１５１份样品的检测说明平板免疫溶血试验与ＥＬＩＳＡ具有很高的符合性。此外对不同来源的红血球用于测定ＬＴ进行了研究。

Heat-labile enterotoxin ( LT)of enterotoxigenic E.coli(ETEC ) from various origins was detected in plateimmune haemotocytolysis assay( PIHA),The results showed that PIHA was higher specifity.The sensitivity of PIHA was similar to ELISA and higher than ligation of rabbit ileum,The analysis of 151 E.coli samples from various origins howed that PIHA was in accord with ELISA。