n3系脂肪酸影响花生四烯酸代谢的研究

THE EFFECTS OF N-3 SERIES FATTY ACIDS ON THE METABOLISM OF ARACHIDONIC ACID

本实验采用洗涤人血小板和人脐动脉环孵育技术,观察二十碳五烯酸(EPA,20:5n3)和二十二碳六烯酸(DHA,22:6n3)对外源性标记花生四烯酸(AA,20:4n6)在血小板和动脉壁内代谢转化的影响。结果表明,EPA和DHA对血小板中血栓素B_2的生成和动脉壁6-Keto-PGF_(1a)的生成均产生剂量依赖性抑制。EPA在低浓度(62.5μM)时可引起血小板生成脂氧化酶产物HETE增加,随EPA浓度升高,HETE产量的增加率降低,甚至在高浓度(1000μM)时表现出HETE生成的抑制,但不显著。DHA除在62.5μM这一浓度时使HETE增加不显著外,其余浓度则以剂量依赖式显著增加HETE的生成。本文结合先前的有关研究资料,就本实验结果进行了讨论。

The effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the arachidonic acid(AA) metabolism were examined by incubating separately washed human platelets and human umbilical artery rings with labelled AA. The results are as follows:1.Both EPA and DHA reduced the formations of TXB2 in platelets and 6-keto-PGF1a in artery walls in dose-dependent way.2.The effect of EPA on the synthesis of HETE by platelets was different with the concentrations of EPA. At lower concentration (62.5μM), it increased the formation of HETE, but the degree of increase was lowerd when the concentration of EPA was increased further, and even reduced the formation of HETE when its concentration reached 1000μM although the decrease extent was statistically insignificant.3.At all of concentrations used but 62.5μM, DHA raised potently the formation of HETE by platelets in dose-dependent way.A brief discussion was made about our results with special references to former data reported.