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鸡卵黄抗体对产肠毒素埃希氏大肠杆菌纤毛的亲和力 被引量:2

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摘要 利用产毒素埃希氏大肠杆菌灭活苗或提纯K99毛免疫鸡和奶捉后制备鸡卵黄抗体,奶牛血清抗体和乳清抗体,用ELISA竞争法测定鸡或牛体内产生的抗K99纤毛抗体的亲和力,卵黄抗体能对免疫奶牛血清和乳清抗体产生强烈的竞争,抑制40-80%抗体的吸附,结果表明免疫鸡抗体与免疫奶牛抗体的亲和力有差异,因此,免疫鸡卵黄抗体,除了可预防某些传染病外,还可通过亲和层析法提纯某些生物活性物质(如纤毛抗原)的有效配体。
作者 Iiem.,Y 秦云
出处 《国外兽医学(畜禽传染病)》 1995年第1期23-25,共3页
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