单纯疱疹病毒性脑炎的诊断──病毒分离，分子杂交和PCR法的比较

Detection of Herpes Simplex Virus in Cerebrospinal Fluid by Polymerase Chain Reaction and omprison with Viral Isolation and Molecular Hybridization

本文对７份临床冻存诊断为病毒性脑炎和１４份其它中枢神经系统疾病的患者的脑脊液标本，分别采用病毒分离，分子杂支和ＰＣＲ法检测其单纯疱疹病毒（ＨＳＶ）。结果７份冻存标本经用３种技术分别检出ＨＳＶ阳性标本为５、３和２份，１４份对照标本全部阴性，说明ＰＣＲ是３种方法中最敏感的检测技术。全部阳性的ＰＣＲ标本电泳核酸区带均经斑点杂交证实为ＨＳＶ－ＤＮＡ，说明ＰＣＲ的特异性。这项初步研究表明，ＰＣＲ技术在该领域的广泛应用，将使我国病毒性脑炎的诊断和治疗大为改观。

To our knowledge,this is the first study in China correlating polymerase chain reaction (PCR),virus isolation and DNA hybridization in cerebrospinal fluid (CSF) for diagnosis of herpes simplex virus encephalitis (HSE).7 CSF specimens from clinically definate HSE patients and 14 from the patients with other neurological diseases(OND) were involved. Herpes simplex virus (HSV) DNA was detected by PCR in 5 of 7 patients with viral encephalitis and in 3 of the 5 HSV DNA PCR positive patients,HSV-DNA was detectable by dot hybridization,while 2 strain of HSV were isolated from the 5 PCR positive samples.All the samples obtained from patients with OND showed nestive by the 3 techniques.The resulls suggested using PCR instead of virus isolation and DNA hybridazition might be a reliable and practical for early and rapid diagnosis of HSE because of its high sensitivity and specificity.