3种苯胺衍生物对大鼠肝脏S_9组分细胞色素P－448活性诱导的研究

Activity of Cytochrome P-448 in S_9 Fraction of Rat Liver Homogenates Induced by Three Aniline Derivatives

用３种结构相似，但致癌／致突变强度不同的苯胺衍生物处理雄性Ｗｉｓｔａｒ大鼠，观察其对大鼠肝脏Ｓ９组分细胞色素Ｐ－４４８的诱导情况，结果见到有致癌作用的ＭＯＣＡ和ＭＤＡ可明显诱导大鼠肝脏Ｓ９组分细胞色素Ｐ－４４８活性，且强致癌剂ＭＯＣＡ的作用强于弱致癌剂ＭＤＡ；而无致癌作用的Ｄａｐｓｏｎｅ则未显现出诱导细胞色素Ｐ－４４８活性，这与它们的致癌／致突变能力相一致。本研究提示有可能用化学物诱导细胞色素Ｐ－４４８的能力来预测其致癌／致突变性。

Treating with three aniline derivatives(MOCA,MDA, Dapsone),which has similar stracture but with dissimilar carcinogenesis or mutagenesis, to male Wistar rats, the activity of cytochrome p-448 in S9 fraction of rat liver homogenates was observed. The experimental results showed that both MOCA and MDA, which were unequivocal carcinogenic chemicals, significantly induced cytochrome P-448.Moreover, the effect of MOCA was stronger than that of MDA that was consistent with the carcinogenicity of two chemicals, But dapsone which was non-mutagenic or non-carcinogenic chemical could not induced activity of cytochrome P-448.The study suggested that we may screen the carcinogenesis or mutagenesis of chemicals by their ability of induction of cytochrome p-448.