血清脂质过氧化物的快速荧光测定

Speedy Fluorometric Assay of Serum Lipoperoxides

本文对荧光法测定血清中的脂质过氧化物之标准操作程度进行了改进，用正交法获得了最佳的实验条件，并对方法的精密度及准确度进行了评价。

A speedy fluorometric assay is described for determination of lipoperoxides in human serum. We propose simple modifications to standard fluorometric assay, to avoid long and tedious manipulations by eliminating the first precipitation and washing steps, the best experiment conditions can be obtained by orthogonal test of multiple factors. Reproducibility of the assay method was excellent for the double serum sample and the coefficient of variance was 5.8% and 3.16% respectively.The fluorescent response was linear up to 0.4ml serum and recovery of method was from 80% to 115%.